In Homestuck there is a connection between genetics and the online handles used by Sburb players. GCAT is a common presentation of the pairings of bases in the double helix structure of DNA, in Homestuck, sixteen abbreviations of online handles can be derived from two-letter combinations of GCAT. Four of them are used by the eight kids (each one used twice), and the other twelve are used by the trolls.

The cherubs expand upon the genetic pattern from DNA to include RNA, with Calliope's handle, uranianUmbra (UU) and Caliborn's handle, undyingUmbrage (uu), which use the letter U, corresponding to uracil, which replaces thymine (T) when information is copied from DNA to RNA. A connection can also be made between these two handles and genotypes, where dominant genes are represented by uppercase letters and recessive genes are represented by lowercase letters, so that UU would represent being homozygous dominant for a genetic trait, and uu would represent being homozygous recessive for a genetic trait.

At the start of Homestuck, John uses the handle ectoBiologist, thus breaking the pattern, however in flashbacks prior to the change he is shown to use a handle that does fit the pattern (ghostyTrickster) but later changed it to avoid the trolls.

All the post-scratch kids have chumhandle abbreviations that match that of the ectobiological sibling of their corresponding pre-scratch kid: Jane/Jade, Jake/John, Roxy/Dave, Dirk/Rose.

The God Cat is also known as "GCat".

Each of these two-letter pair is the abbreviation used for an online handle of one of the trolls or kids (except for John):

G gardenGnostic [GG]
JadeLogo Jade Harley
gallowsCalibrator [GC]
Libra Terezi Pyrope
grimAuxiliatrix [GA]
Virgo Kanaya Maryam
ghostyTrickster [GT]
JohnLogo John Egbert
gutsyGumshoe [GG]
JaneLogo Jane Crocker
golgothasTerror [GT]
JakeLogo Jake English
C carcinoGeneticist [CG]
Cancer Karkat Vantas
cuttlefishCuller [CC]
Pisces Feferi Peixes
caligulasAquarium [CA]
Aquarius Eridan Ampora
centaursTesticle [CT]
Sagittarius Equius Zahhak
A arachnidsGrip [AG]
Scorpio Vriska Serket
arsenicCatnip [AC]
Leo Nepeta Leijon
apocalypseArisen [AA]
Aries Aradia Megido
adiosToreador [AT]
Taurus Tavros Nitram
T turntechGodhead [TG]
DaveLogo Dave Strider
terminallyCapricious [TC]
Capricorn Gamzee Makara
twinArmageddons [TA]
Gemini Sollux Captor
tentacleTherapist [TT]
RoseLogo Rose Lalonde
tipsyGnostalgic [TG]
RoxyLogo Roxy Lalonde
timaeusTestified [TT]
DirkLogo Dirk Strider
U u
U uranianUmbra [UU]
Calliope symbol Calliope
u undyingUmbrage [uu]
Caliborn symbol Caliborn


  • The kids' combinations do not include the letters A or C. Conversely, all of the trolls' combinations include either A or C.
    • Because of this, the kids' and trolls' portions individually are reflective of the shapes of their respective Computer Terminals when placed in the order GCAT.
  • Of the four pre-scratch kids the boys, John and Dave, have heterozygous handle abbreviations, GT/EB and TG, while the girls, Rose and Jade, have homozygous abbreviations, TT and GG, resembling the heterozygous male XY chromosomes and the homozygous female XX chromosomes. The two remaining same-letter combinations (AA and CC) do belong to female trolls, but the remaining female trolls are GA, GC, AC, and AG; the number of female trolls would be limited to two if the pattern was not broken. This could also be explained by the fact that we currently do not know how chromosomes factor into trolls' reproduction, if at all. Of the post-scratch kids, Jane and Jake continue the pattern having GG and GT, however Dirk and Roxy break it having TT and TG, respectively.
    • Also, the humans have the same handle abbreviation as their respective paradox siblings' guardians' pre/post-scratch counterparts. For Example, TT and TT.